IL-33 Protein (Transcript Variant 1)
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- Target See all IL-33 (IL33) Proteins
- IL-33 (IL33) (Interleukin 33 (IL33))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Interleukin-33 (IL33) (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
- Biological Activity Comment
- The ED50 is 0.05 to 0.25 ng/ml, corresponding to a specific activity of 4 x 10^6 to 2 x 10^7 units/mg, determined by the dose dependent stimulation of anti-CD3 activated D10.G4.1 cell proliferation.
- Top Product
- Discover our top product IL33 Protein
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Interleukin 33 (IL33) (AA 112-270) protein
IL33 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 33 (IL33) (AA 109-270) (Active) protein (His tag)IL33 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 33 (IL33) (AA 109-270) protein (His tag)IL33 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 33 (IL33) (AA 109-264) protein (His tag)IL33 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 33 (IL33) (AA 1-270) protein (GST tag)IL33 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 33 (IL33) (AA 171-269) protein (GST tag)IL33 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 33 (IL33) protein (His tag)IL33 Origin: Rhesus Monkey Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 1 x PBS
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- IL-33 (IL33) (Interleukin 33 (IL33))
- Alternative Name
- Interleukin-33 (Il33) (IL33 Products)
- Synonyms
- IL33 Protein, C9orf26 Protein, DVS27 Protein, IL1F11 Protein, NF-HEV Protein, NFEHEV Protein, RP11-575C20.2 Protein, 9230117N10Rik Protein, Il-33 Protein, Il1f11 Protein, RGD1311155 Protein, interleukin 33 Protein, IL33 Protein, Il33 Protein
- Background
- The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 18 kDa
- NCBI Accession
- NP_254274
- Pathways
- Production of Molecular Mediator of Immune Response
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