KMO Protein (Myc-DYKDDDDK Tag)
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- Target See all KMO Proteins
- KMO (Kynurenine 3-Monooxygenase (Kynurenine 3-Hydroxylase) (KMO))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This KMO protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Kynurenine 3-monooxygenase / KMO protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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Development of a Surface Plasmon Resonance Assay for the Characterization of Small-Molecule Binding Kinetics and Mechanism of Binding to Kynurenine 3-Monooxygenase." in: Assay and drug development technologies, Vol. 13, Issue 8, pp. 466-75, (2015) (PubMed).
: "
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Development of a Surface Plasmon Resonance Assay for the Characterization of Small-Molecule Binding Kinetics and Mechanism of Binding to Kynurenine 3-Monooxygenase." in: Assay and drug development technologies, Vol. 13, Issue 8, pp. 466-75, (2015) (PubMed).
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- Target
- KMO (Kynurenine 3-Monooxygenase (Kynurenine 3-Hydroxylase) (KMO))
- Alternative Name
- Kynurenine 3-Monooxygenase,kmo (KMO Products)
- Synonyms
- AI046660 Protein, dJ317G22.1 Protein, kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) Protein, kynurenine 3-monooxygenase Protein, Kmo Protein, KMO Protein
- Background
- Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 55.6 kDa
- NCBI Accession
- NP_003670
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