Mitochondrial Ribosomal Protein L43 (MRPL43) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2726356, Supplier: Log in to see
Protein Name
  • bMRP36a
  • 4930442D21Rik
  • mitochondrial ribosomal protein L43
  • MRPL43
  • Mrpl43
Protein Characteristics
Transcript Variant 1
1
1
1
Origin
Human
2
Source
HEK-293 Cells
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This MRPL43 protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
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Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human MRPL43 (transcript variant 1) protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms.
Molecular Weight 17.7 kDa
NCBI Accession NP_115488
Research Area DNA/RNA, Chromatin and Nuclear Signaling, Organelles
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Western Blotting (WB) image for Mitochondrial Ribosomal Protein L43 (MRPL43) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag) (ABIN2726356) Validation with Western Blot
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