Nucleolin Protein (NCL) (Myc-DYKDDDDK Tag)
(3 references)-
- Target See all Nucleolin (NCL) Proteins
- Nucleolin (NCL)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Nucleolin protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Nucleolin protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NCL Protein
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Nucleolin (NCL) (AA 2-710) protein (His tag)
Crystallography grade NCL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Nucleolin (NCL) (AA 2-707) protein (His tag)Crystallography grade NCL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Nucleolin (NCL) (AA 2-482), (partial) protein (His tag)NCL Origin: Human Host: Yeast Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Nucleolin promotes in vitro translation of feline calicivirus genomic RNA." in: Virology, Vol. 489, pp. 51-62, (2016) (PubMed).
: "The G-quadruplex-forming aptamer AS1411 potently inhibits HIV-1 attachment to the host cell." in: International journal of antimicrobial agents, Vol. 47, Issue 4, pp. 311-6, (2016) (PubMed).
: "Nucleolin stabilizes G-quadruplex structures folded by the LTR promoter and silences HIV-1 viral transcription." in: Nucleic acids research, Vol. 43, Issue 18, pp. 8884-97, (2015) (PubMed).
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: "Nucleolin promotes in vitro translation of feline calicivirus genomic RNA." in: Virology, Vol. 489, pp. 51-62, (2016) (PubMed).
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- Target
- Nucleolin (NCL)
- Alternative Name
- Nucleolin (NCL Products)
- Synonyms
- C23 Protein, B530004O11Rik Protein, D0Nds28 Protein, D1Nds28 Protein, Nucl Protein, wu:fa12d03 Protein, zgc:152810 Protein, nucl Protein, LOC100282923 Protein, LOC100283058 Protein, nucleolin Protein, nucleolin S homeolog Protein, NCL Protein, Ncl Protein, ncl.S Protein, ncl Protein, LOC100195537 Protein, LOC100282923 Protein, LOC100283058 Protein
- Background
- Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
- Molecular Weight
- 76.4 kDa
- NCBI Accession
- NP_005372
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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