POLR3K Protein (Myc-DYKDDDDK Tag)
-
- Target See all POLR3K Proteins
- POLR3K (Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POLR3K protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human POLR3K protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product POLR3K Protein
-
Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K) (AA 1-108) protein (His tag)
POLR3K Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K) (AA 1-108) protein (GST tag)POLR3K Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K) (AA 1-108) protein (Strep Tag)Crystallography grade POLR3K Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K) (AA 1-108) protein (Strep Tag)Crystallography grade POLR3K Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K) (AA 1-108), (full length) protein (His-SUMO Tag)POLR3K Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- POLR3K (Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa (POLR3K))
- Alternative Name
- Polr3k (POLR3K Products)
- Synonyms
- MGC108467 Protein, POLR3K Protein, 12.3kDa Protein, 1500004O14Rik Protein, AI196849 Protein, AU014893 Protein, C11 Protein, RPC10 Protein, RPC11 Protein, C11-RNP3 Protein, RPC12.5 Protein, hRPC11 Protein, RNA polymerase III subunit K Protein, polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa S homeolog Protein, polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa Protein, polymerase (RNA) III (DNA directed) polypeptide K Protein, Polr3k Protein, polr3k.S Protein, polr3k Protein, POLR3K Protein
- Background
- This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010].
- Molecular Weight
- 12.1 kDa
- NCBI Accession
- NP_057394
-
