RPE65 Protein (Myc-DYKDDDDK Tag)
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- Target See all RPE65 Proteins
- RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RPE65 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human RPE65 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product RPE65 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))
- Alternative Name
- Rpe65 (RPE65 Products)
- Synonyms
- LCA2 Protein, RP20 Protein, mRPE65 Protein, rd12 Protein, sRPE65 Protein, 65kDa Protein, A930029L06Rik Protein, Mord1 Protein, DRPE65 Protein, RPE65 Protein, anon-WO0148162.1 Protein, anon-WO0148163.1 Protein, beta-diox Protein, DmelCG9347 Protein, CG9347 Protein, MGC52887 Protein, MGC154296 Protein, MGC85437 Protein, rpe65 Protein, RPE56c Protein, si:ch211-198n5.12 Protein, 13cIMH Protein, RPE56b Protein, rpepb Protein, si:ch211-198n5.5 Protein, wu:fc74c09 Protein, zgc:110538 Protein, RPE56a Protein, rpepa Protein, zgc:73213 Protein, RPE65, retinoid isomerohydrolase Protein, retinal pigment epithelium 65 Protein, neither inactivation nor afterpotential B Protein, retinal pigment epithelium-specific protein 65kDa S homeolog Protein, retinal pigment epithelium-specific protein 65kDa L homeolog Protein, RPE65 homolog Protein, retinal pigment epithelium-specific protein 65kDa Protein, retinal pigment epithelium-specific protein 65c Protein, retinal pigment epithelium-specific protein 65b Protein, retinal pigment epithelium-specific protein 65a Protein, RPE65 Protein, Rpe65 Protein, ninaB Protein, rpe65.S Protein, rpe65.L Protein, rpe65 Protein, rpe65c Protein, rpe65b Protein, rpe65a Protein
- Background
- This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.
- Molecular Weight
- 60.8 kDa
- NCBI Accession
- NP_000320
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