SPAG1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2732486

Product Details

Target: SPAG1 (Sperm Associated Antigen 1 (SPAG1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SPAG1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human SPAG1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SPAG1 (Sperm Associated Antigen 1 (SPAG1))

Alternative Name: Spag1 (SPAG1 Products)

Synonyms: cb1089 Protein, MGC162178 Protein, wu:fj78g10 Protein, sp75 Protein, tpis Protein, hsd-3.8 Protein, HSD-3.8 Protein, SP75 Protein, TPIS Protein, sperm associated antigen 1 Protein, sperm associated antigen 1a Protein, SPAG1 Protein, spag1a Protein, CpipJ_CPIJ006297 Protein, spag1 Protein, Spag1 Protein

Background: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.

Molecular Weight: 103.5 kDa

NCBI Accession: NP_003105