SPI1 Protein (His tag)
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- Target See all SPI1 Proteins
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SPI1 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human SPI1 / PU.1 (N-term HIS tag, transcript variant 1) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product SPI1 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
- Alternative Name
- Spi1,pu.1 (SPI1 Products)
- Synonyms
- OF Protein, PU.1 Protein, SFPI1 Protein, SPI-1 Protein, SPI-A Protein, Dis-1 Protein, Dis1 Protein, Sfpi-1 Protein, Sfpi1 Protein, Spi-1 Protein, Tcfpu1 Protein, Tfpu.1 Protein, Pu.1 Protein, cb1086 Protein, pu.1 Protein, pu1 Protein, sfpi1 Protein, si:by184l24.2 Protein, spi1 Protein, SPI1/PU.1 Protein, spi1l Protein, zgc:152991 Protein, Spi-1 proto-oncogene Protein, spleen focus forming virus (SFFV) proviral integration oncogene Protein, Spi-1 proto-oncogene b Protein, Spi-1 proto-oncogene a Protein, SPI1 Protein, Spi1 Protein, spi1b Protein, spi1a Protein
- Background
- This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 31 kDa
- NCBI Accession
- NP_001074016
- Pathways
- Stem Cell Maintenance
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