TTL Protein (Myc-DYKDDDDK Tag)
-
- Target See all TTL Proteins
- TTL (Tubulin tyrosine Ligase (TTL))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TTL protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human TTL protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product TTL Protein
-
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- TTL (Tubulin tyrosine Ligase (TTL))
- Alternative Name
- Ttl (TTL Products)
- Synonyms
- 2410003M22Rik Protein, 2700049H19Rik Protein, AI848570 Protein, wu:fb93a09 Protein, zgc:153332 Protein, tubulin tyrosine ligase Protein, Ttl Protein, TTL Protein, ttl Protein
- Background
- TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM, Mar 2008].
- Molecular Weight
- 43 kDa
- NCBI Accession
- NP_714923
- Pathways
- Regulation of Cell Size
-