KARS Protein (AA 2-597) (His tag)

Catalog No. ABIN3095758

Product Details

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Protein Type: Recombinant

Protein Characteristics: AA 2-597

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This KARS protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: AAVQAAEVKV DGSEPKLSKN ELKRRLKAEK KVAEKEAKQK ELSEKQLSQA TAAATNHTTD NGVGPEEESV DPNQYYKIRS QAIHQLKVNG EDPYPHKFHV DISLTDFIQK YSHLQPGDHL TDITLKVAGR IHAKRASGGK LIFYDLRGEG VKLQVMANSR NYKSEEEFIH INNKLRRGDI IGVQGNPGKT KKGELSIIPY EITLLSPCLH MLPHLHFGLK DKETRYRQRY LDLILNDFVR QKFIIRSKII TYIRSFLDEL GFLEIETPMM NIIPGGAVAK PFITYHNELD MNLYMRIAPE LYHKMLVVGG IDRVYEIGRQ FRNEGIDLTH NPEFTTCEFY MAYADYHDLM EITEKMVSGM VKHITGSYKV TYHPDGPEGQ AYDVDFTPPF RRINMVEELE KALGMKLPET NLFETEETRK ILDDICVAKA VECPPPRTTA RLLDKLVGEF LEVTCINPTF ICDHPQIMSP LAKWHRSKEG LTERFELFVM KKEICNAYTE LNDPMRQRQL FEEQAKAKAA GDDEAMFIDE NFCTALEYGL PPTAGWGMGI DRVAMFLTDS NNIKEVLLFP AMKPEDKKEN VATTDTLEST TVGTSV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human KARS Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: KARS (KARS Products)

Synonyms: CG12141 Protein, Dmel\\CG12141 Protein, KRS Protein, LysRS Protein, cb530 Protein, wu:fa16h02 Protein, zgc:92483 Protein, kars Protein, MGC53345 Protein, kars2 Protein, krs Protein, krs-1 Protein, KARS Protein, Tb06.28P18.190 Protein, CMTRIB Protein, DFNB89 Protein, KARS1 Protein, KARS2 Protein, AA589550 Protein, AL024334 Protein, AL033315 Protein, AL033367 Protein, D8Ertd698e Protein, D8Wsu108e Protein, mKIAA0070 Protein, Lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase S homeolog Protein, lysyl-tRNA synthetase L homeolog Protein, LysRS Protein, kars Protein, kars.S Protein, kars.L Protein, Bm1_16500 Protein, lysS Protein, Taci_1118 Protein, Tpau_1737 Protein, Arch_0261 Protein, Trad_2960 Protein, Spirs_3699 Protein, KARS Protein, LOC100280510 Protein, Tb927.6.1510 Protein, Kars Protein

Background: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. {ECO:0000269|PubMed:15851690, ECO:0000269|PubMed:5338216}.

Molecular Weight: 68.9 kDa Including tag.

UniProt: Q15046