Cell Division Cycle Associated 7-Like (CDCA7L) (AA 1-438) protein (His tag)

Details for Product No. ABIN3127713, Supplier: Log in to see
Protein Name
  • ram2
  • MGC154777
  • BC006933
  • JPO2
  • R1
  • RAM2
  • RAPGEF5
  • cell division cycle associated 7-like
  • cell division cycle associated 7 like
  • CDCA7L
  • cdca7l
  • LOC100348574
  • Cdca7l
Protein Characteristics
AA 1-438
2
1
1
Origin
Mouse (Murine)
2
1
Source
Insect Cells
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
His tag
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Sequence MELATRSQIP KEVADIFSAP SDDEEFVGFQ DDVPMQNLSE SCGSLDSREL EKQQNVCFRS KYFTEELRRI FKEDTDSEME DFEGFTESEL NMSSNPELME SELSDSDKAY PVMNDAEEDD EEEAAPRRGR STRRSSFGLR VAFQFPTKKL ARTPDKDSSH LLDSKTDLRR KKSSRQPKGK EDSASDAEDE SRAESQENSD ALLKRAMNIK ENKAMLAQLL AELNSVPDFF PVRTPPSASR RRTPRRAFSE GQITRRMNPT RSARPPEKFA LENFTFSATK LTEELYSFRR RKTISGGKCQ TYRRHRISSF RSVKDITEED LENIAITVRD KVYDKVLGNT CHQCRQKTID TKTVCRNQSC GGVRGQFCGP CLRNRYGEDV RTALLDPKWT CPPCRGICNC SYCRRRDGRC ATGILIHLAK FYGYDNVKEY LESLQKQL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Cdca7l Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Background Plays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. Plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells (By similarity). Involved in apoptotic signaling pathways, May act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1. {ECO:0000250, ECO:0000269|PubMed:16829576}.
Molecular Weight 51.2 kDa Including tag.
UniProt Q922M5
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Cell Division Cycle Associated 7-Like (CDCA7L) (AA 1-438) protein (His tag) (ABIN3127713) „Crystallography Grade“ protein due to multi-step, protein-specific purification process