NUP50 Protein (AA 1-466) (Strep Tag)

Catalog No. ABIN3130065

Product Details

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Protein Type: Recombinant

Protein Characteristics: AA 1-466

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This NUP50 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MAKRVAEKEL TDRNWDEEDE VEEMGTFSVA SEEVMKNRAV KKAKRRNVGF ESDSGGAFKG FKGLVVPSGG GGFSGFGGSG GKPLEGLTNG NSTDNATPFS NVKTAAEPKA AFGSFAVNGP TTLVDKKISS PKCNNSNQPP SSGPASSTAC PGNAYHKQLA GLNCSVRDWI VKHVNTNPLC DLTPIFKDYE RYLATIEKQL ENGGGSSSES QTDRATAGME PPSLFGSTKL QQESPFSFHG NKAEDTSEKV EFTAEKKSDA AQGATSASFS FGKKIESSAL GSLSSGSLTG FSFSAGSSSL FGKDAAQSKA ASSLFSAKAS ESPAGGGSSE CRDGEEEEND EPPKVVVTEV KEEDAFYSKK CKLFYKKDNE FKEKGVGTLH LKPTATQKTQ LLVRADTNLG NILLNVLIAP NMPCTRTGKN NVLIVCVPNP PLDEKQPTLP ATMLIRVKTS EDADELHKIL LEKKDA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Alternative Name: Nup50 (NUP50 Products)

Synonyms: wu:fa56e03 Protein, wu:fb78a08 Protein, GB18194 Protein, npap60 Protein, npap60l Protein, Nup50 Protein, Rtp60 Protein, 1700030K07Rik Protein, AI413123 Protein, Npap60 Protein, NPAP60 Protein, NPAP60L Protein, nucleoporin 50 Protein, nuclear pore complex protein Nup50 Protein, nucleoporin 50kDa S homeolog Protein, nucleoporin 50kDa Protein, nup50 Protein, LOC410864 Protein, NUP50 Protein, nup50.S Protein, CpipJ_CPIJ012737 Protein, Nup50 Protein

Background: Nuclear pore complex protein Nup50 (50 kDa nucleoporin) (Nuclear pore-associated protein 60 kDa-like) (Nucleoporin Nup50),FUNCTION: Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:10811608). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:16222336). Interacts with regulatory proteins of cell cycle progression including CDKN1B (PubMed:10891500, PubMed:10811608). This interaction is required for correct intracellular transport and degradation of CDKN1B (PubMed:10811608). {ECO:0000269|PubMed:10811608, ECO:0000269|PubMed:10891500, ECO:0000269|PubMed:16222336}.

Molecular Weight: 49.5 kDa

UniProt: Q9JIH2

Pathways: Tube Formation