phosphofurin Acidic Cluster Sorting Protein 2 (PACS2) (AA 1-862) protein (His tag)

Details for Product No. ABIN3135128, Supplier: Log in to see
Protein Name
  • PACS-2
  • PACS1L
  • 6720425G15Rik
  • AW208651
  • AW489976
  • E230011J22
  • Pacs1l
  • mKIAA0602
  • phosphofurin acidic cluster sorting protein 2
  • PACS2
  • Pacs2
Protein Characteristics
AA 1-862
2
1
1
Origin
Mouse (Murine)
3
Source
Insect Cells
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This PACS2 protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Sequence MAERGRLGLP GAPGALNTPV PMNLFATWEV DGSSPSCVPR LCSLTLKKLA VLRELEKELL SVVIAVKMQY PHFLKREGNK LQIMLQRRKR YKNRTILGYK TLAAGSINMA EVMQHPSEGG QVLSLCSSIK EASVKVAEIW IVSLSSQPID HEDSAMQAGP KTKSTDNYSE EEYESFSSEQ EASDDAVQGQ DLDEDDFDVG KPKKQRRSIV RTTSMTRQQN FKQKVVALLR RFKVSEEVLD SEQDPAEHVP EVEEDLDLLY DTLDVENPSD SGPDMDDDDS VLSTPKPKLR PYFEGLSHSS SQTEIGSIHS ARSHREPPSP ADVPEKTRSL GGKQQLSDSV SDTVALSAAV PREPSGQPED SPEAETSTLD VFTEKLPPSG RIIKTESLVI PSTRSESKPA GRRGRSTSLK ERQPARPQNE RANSLDNERC PDTRSQLQIP RKTVYDQLNH ILISDDQLPE NIILVNTSDW QGQFLSDVLQ KHTLPVVCTC SAADVQAAFS TIVSRIQRYC NCNSQPPTPV KIAVAGAQHY LSAILRLFVE QLSHKTPDWL GYMRFLIIPL GSHPVARYLG SVDYRYNNFF QDLAWRDLFN KLEAQSSVQD TPDIVSRITQ YISGANCAHQ LPIAEAMLTY KQKSPDEESS QRFIPFVGVV KVGIVEPSSA TSGDSDDAAP SSSSILSSTP PSASTSPAAK EASPTPPSSP SVSGGLSSPS QGVGAELMGL QVDYWTAAQP ADRKRDAEKK DMPTTKNTLK CTFRSLQVSR LPSSGEAAAT PTMSMTVVTK EKNKKVMFLP KKTKDKEVES KSQCIEGISR LICTAKHQQN MLRVLIDGVE CSDVKFFQLA AQWSSHVKHF PICIFGHSKA TF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Pacs2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Background Multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, including the apposition of mitochondria with the ER and ER homeostasis. In addition, in response to apoptic inducer, translocates BIB to mitochondria, which initiates a sequence of events including the formation of mitochondrial truncated BID, the release of cytochrome c, the activation of caspase-3 thereby causing cell death. May also involved in ion channel trafficking, directing acidic cluster-containing ion channels to distict subcellular compartments (By similarity). {ECO:0000250}.
Molecular Weight 95.9 kDa Including tag.
UniProt Q3V3Q7
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for phosphofurin Acidic Cluster Sorting Protein 2 (PACS2) (AA 1-862) protein (His tag) (ABIN3135128) „Crystallography Grade“ protein due to multi-step, protein-specific purification process