KDM3B Protein (AA 2-1562) (His tag)

Catalog No. ABIN3135870

Product Details

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Protein Type: Recombinant

Protein Characteristics: AA 2-1562

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This KDM3B protein is labelled with His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequence: ADAAASPVGK RLLLLFADPT ASASASAPTA AAVVSGDPGP ALRTRAWRAG TVRAMSGAVP QDLAIFVEFD GCNWKQHSWV KVHAEDVLAL LLEGSLVWAP RKDPVLLQGT RVPVAQWPAL TFTPLVDKLG LGSVVPVEYL VDRELRFLSD ANGMHLFQMG TDVQNQILLE HAALRETVNA LISDQKLQEI FSRGPYSVQG HRVKVYQPEG EEVWLCGVVS RQDSVTRLME VSITETGEVK SVDPRLTHVM LMDSSTPQSE NSRNSSLASS GFGVSLSSLS QPLTFGSGRS QSNGVLATDN KPLGFSFSCS SASESQKDSD LSKNLFFQCM SQNVPSTNYL SRVSESVADD SSSRDSFTQS LESLTSGLCK GRSVLGADTQ PGPKAGSSVD RKVPAESMPT LTPAFPRSLL NTRTPENHEN LFLQPPKLSR EEPSNPFLAF VEKVEHSPFS SFVSQASGSS SSATSVTSKA TASWPESHSS AESAPLAKKK PLFITTDSSK LVSGVLGSAL STGSPSLSAV GNGRSSSPTN SLTQPIEMPT LSSSPTEERP TVGPGQQDNP LLKTFSTVFG RHSGSFLSAP AEFAQENKAP FEAVKRFSLD ERSLACRQDS DSSTNSDLSD LSDSEEQLQA KSGLKGIPEH LMGKLGPNGE RSAELLLGKG KGKQAPKGRP RTAPLKVGQS VLKDVSKVRK LKQSGEPFLQ DGSCINVAPH LHKCRECRLE RYRKFKEQEQ DDSTVACRFF HFRRLVFTRK GVLRVEGFLS PQQSDPDAMN LWIPSSSLAE GIDLETSKYI LANVGDQFCQ LVMSEKEAMM MVEPHQKVAW KRAVRGVREM CDVCETTLFN IHWVCRKCGF GVCLDCYRLR KSRPRSETEE MGDEEVFSWL KCAKGQSHEP ENLMPTQIIP GTALYNIGDM VHAARGKWGI KANCPCISRQ SKSVLRPAVT NGISQLPSVT PSASSGNETT FSSGGGAAAV TNPEPDQVPK GAGTDGRSEE PLKAEGSASN SNSELKAIRP PCPDTAPPSS ALHWLADLAT QKAKEETKDA GSLRSVLNKE SHSPFGLDSF NSTAKVSPLT PKLFNSLLLG PTASNSKTEG SSLRDLLHSG PGKLPQTPLD TGIPFPPVFS SSSAVAKSKA SLPDFLDHII ASVVENKKTS DPSKRSCNLT DTQKEVKEMA MGLNVLDPHT SHSWLCDGRL LCLHDPSNKN NWKIFRECWK QGQPVLVSGV HKKLKSELWK PEAFSQEFGD QDVDLVNCRN CAIISDVKVR DFWDGFEIIC KRLRSEDGQP MVLKLKDWPP GEDFRDMMPT RFEDLMENLP LPEYTKRDGR LNLASRLPSY FVRPDLGPKM YNAYGLITAE DRRVGTTNLH LDVSDAVNVM VYVGIPVGEG AHDEEVLKTI DEGDADEVTK QRIHDGKEKP GALWHIYAAK DAEKIRELLR KVGEEQGQEN PPDHDPIHDQ SWYLDQILRK RLFEEYGVQG WAIVQFLGDA VFIPAGAPHQ VHNLYSCIKV AEDFVSPEHV KHCFRLTQEF RHLSNTHTNH EDKLQVKNII YHAVKDAVGT LKAHESKLAR S
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Kdm3b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Alternative Name: Kdm3b (KDM3B Products)

Synonyms: KDM3B Protein, JMJD1B Protein, 5830462I21Rik Protein, JHDM2B Protein, Jmjd1b Protein, mKIAA1082 Protein, 5qNCA Protein, C5orf7 Protein, NET22 Protein, lysine demethylase 3B Protein, lysine (K)-specific demethylase 3B Protein, lysine-specific demethylase 3B Protein, KDM3B lysine (K)-specific demethylase 3B Protein, KDM3B Protein, LOC100540242 Protein, kdm3b Protein, Kdm3b Protein

Background: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate May have tumor suppressor activity (By similarity). {ECO:0000250}.

Molecular Weight: 171.7 kDa Including tag.

UniProt: Q6ZPY7

Pathways: Warburg Effect