SMAD1 Protein (AA 1-465) (GST tag)

Catalog No. ABIN7313592

Product Details

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Protein Type: Recombinant

Protein Characteristics: AA 1-465

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SMAD1 protein is labelled with GST tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human SMAD1 Protein is produced by our E. coli expression system and the target gene encoding Met1-Ser465 is expressed with a GST tag at the N-terminus.

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMNV TSLFSFTSPA VKRLLGWKQG DEEEKWAEKA VDALVKKLKK KKGAMEELEK ALSCPGQPSN CVTIPRSLDG RLQVSHRKGL PHVIYCRVWR WPDLQSHHEL KPLECCEFPF GSKQKEVCIN PYHYKRVESP VLPPVLVPRH SEYNPQHSLL AQFRNLGQNE PHMPLNATFP DSFQQPNSHP FPHSPNSSYP NSPGSSSSTY PHSPTSSDPG SPFQMPADTP PPAYLPPEDP MTQDGSQPMD TNMMAPPLPS EINRGDVQAV AYEEPKHWCS IVYYELNNRV GEAFHASSTS VLVDGFTDPS NNKNRFCLGL LSNVNRNSTI ENTRRHIGKG VHLYYVGGEV YAECLSDSSI FVQSRNCNYH HGFHPTTVCK IPSGCSLKIF NNQEFAQLLA QSVNHGFETV YELTKMCTIR MSFVKGWGAE YHRQDVTSTP CWIEIHLHGP LQWLDKVLTQ MGSPHNPISS VS

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1 (SMAD1 Products)

Synonyms: MLP Protein, Xmad1 Protein, Xsmad1 Protein, bsp1 Protein, jv4-1 Protein, jv41 Protein, madh1 Protein, madr1 Protein, smad-1 Protein, MADH1 Protein, fb39h09 Protein, wu:fb39h09 Protein, wu:fb97e04 Protein, 2/23 Protein, CG12399 Protein, Dmel\\CG12399 Protein, E(zen)2 Protein, En(vvl) Protein, MAD Protein, Mat Protein, P-Mad Protein, P-mad Protein, PMad Protein, Smad Protein, Smad1 Protein, apg Protein, c28 Protein, dMAD Protein, dMad Protein, l(2)K00237 Protein, l(2)k00237 Protein, mad Protein, p-Mad Protein, pMAD Protein, pMad Protein, pSmad Protein, BSP-1 Protein, BSP1 Protein, JV4-1 Protein, JV41 Protein, MADR1 Protein, AI528653 Protein, Madh1 Protein, Madr1 Protein, Mlp1 Protein, MusMLP Protein, smad1 Protein, xmad Protein, SMAD family member 1 Protein, Mothers against dpp Protein, SMAD family member 1 L homeolog Protein, smad1 Protein, SMAD1 Protein, Mad Protein, Smad1 Protein, smad1.L Protein

Gene ID: 4086

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development