Ube2t Protein (AA 1-197) (His tag)
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- Target See all Ube2t Proteins
- Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-197
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Ube2t protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)
- Purpose
- Recombinant Human UBE2T Protein is produced by our E. coli expression system and the target gene encoding Met1-Val197 is expressed with a 6His tag at the N-terminus.
- Sequence
- MGSSHHHHHH SSGLVPRGSH MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDV
- Purity
- Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product Ube2t Protein
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Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) protein (His tag)
Ube2t Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) protein (GST tag)Ube2t Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) protein (Strep Tag)Crystallography grade Ube2t Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ubiquitin-Conjugating Enzyme E2T (Ube2t) protein (Myc-DYKDDDDK Tag)Ube2t Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))
- Alternative Name
- UBE2T (Ube2t Products)
- Synonyms
- PIG50 Protein, UBE2T Protein, 2700084L22Rik Protein, C80607 Protein, RGD1310816 Protein, hspc150 Protein, wu:fj86h11 Protein, zgc:153687 Protein, ubiquitin conjugating enzyme E2 T Protein, ubiquitin-conjugating enzyme E2T Protein, ubiquitin conjugating enzyme E2T S homeolog Protein, ubiquitin-conjugating enzyme E2T (putative) Protein, UBE2T Protein, Ube2t Protein, ube2t.S Protein, ube2t Protein
- Gene ID
- 29089
- UniProt
- Q9NPD8
- Pathways
- Chromatin Binding
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