WNT3A Protein (AA 19-352)
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- Target See all WNT3A Proteins
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 19-352
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Origin
- Human
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Source
- Mammalian Cells
- Application
- Functional Studies (Func)
- Specificity
- Stem cell or organoid culture
- Purification
- This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
- Purity
- 90-95 %
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg)
- Biological Activity Comment
- Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 17Feb2016) generate 50- fold increase of luciferase activity compared to control (buffer without Wnt3a).
- Top Product
- Discover our top product WNT3A Protein
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Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 1-385) protein (GST tag)
WNT3A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-352) protein (His-IF2DI Tag)WNT3A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-351), (partial) protein (His tag)WNT3A Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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30-60 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
- Handling Advice
- Mix the protein by pipetting up and down but not by vortexing
- Storage
- -20 °C
- Storage Comment
- Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
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- Target
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
- Alternative Name
- Wnt3a (WNT3A Products)
- Synonyms
- WNT3A Protein, Wnt-3a Protein, vt Protein, Zwnt[a] Protein, wnt3 Protein, wnt3 l Protein, wnt3l Protein, wnt[a] Protein, Xwnt-3a Protein, wnt-3a Protein, wnt3a-A Protein, xwnt3a Protein, WNT-3A Protein, WNT3 Protein, wingless-type MMTV integration site family, member 3A Protein, Wnt family member 3A Protein, wingless-type MMTV integration site family member 3A L homeolog Protein, WNT3A Protein, Wnt3a Protein, wnt3a Protein, wnt3a.L Protein
- Background
- Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Human Wnt3a shows 96 % amino acid identity to mouse Wnt3a protein
- Molecular Weight
- 41 kDa evaluated by SDS-PAGE under reducing conditions
- Gene ID
- 89780
- UniProt
- P56704
- Pathways
- WNT Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
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