RASGRP3 Protein (GST tag,His tag)
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- Target See all RASGRP3 Proteins
- RASGRP3 (RAS Guanyl Releasing Protein 3 (Calcium and DAG-Regulated) (RASGRP3))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RASGRP3 protein is labelled with GST tag,His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Purified recombinant protein of Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50 μg (full length, N-term GST tag, C-term His tag, transcript variant 2) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purification
- Purified
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product RASGRP3 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal,N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- RASGRP3 (RAS Guanyl Releasing Protein 3 (Calcium and DAG-Regulated) (RASGRP3))
- Alternative Name
- RAS guanyl releasing protein 3 (RASGRP3 Products)
- Synonyms
- RASGRP3 Protein, zgc:162151 Protein, GRP3 Protein, BC066069 Protein, Gm327 Protein, RAS guanyl releasing protein 3 Protein, RAS guanyl releasing protein 3 (calcium and DAG-regulated) Protein, RAS, guanyl releasing protein 3 Protein, RASGRP3 Protein, rasgrp3 Protein, Rasgrp3 Protein
- Background
- Members of the RAS (see HRAS MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008].
- Molecular Weight
- 104.2 kDa
- NCBI Accession
- NP_733772
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