LILRB3 Protein (AA 24-443) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN5526652

Product Details

Target: LILRB3 (Leukocyte Immunoglobulin-Like Receptor B3 (LILRB3))

Protein Type: Recombinant

Protein Characteristics: AA 24-443

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LILRB3 protein is labelled with Fc Tag,AVI tag,Biotin.

Brand: PrecisionAvi

Sequence: AA 24-443

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 74.5 kDa. As a result of glycosylation, the protein migrates as 80-105 KDa under reducing (R) condition, and 150 KDa under non-reducing (NR) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: LILRB3 (Leukocyte Immunoglobulin-Like Receptor B3 (LILRB3))

Alternative Name: LILRB3 (LILRB3 Products)

Synonyms: CD85A Protein, HL9 Protein, ILT-5 Protein, ILT5 Protein, LILRA6 Protein, LIR-3 Protein, LIR3 Protein, PIRB Protein, Gp91 Protein, Lilrb3 Protein, PIR-B Protein, Pirb Protein, NILR-1 Protein, LILRB3 Protein, leukocyte immunoglobulin like receptor B3 Protein, paired Ig-like receptor B Protein, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 Protein, LILRB3 Protein, Pirb Protein

Background: Leukocyte immunoglobulin-like receptor subfamily B member 3 is also known as LILRB3,ILT-5 or CD85a. LILRB3 plays an role as receptor for class I MHC antigens, which activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. LILRB3 and LILRA6 represent a pair of inhibitory/activating receptors with identical extracellular domains and unknown ligands. LILRB3 can mediate inhibitory signaling via immunoreceptor tyrosine-based inhibition motifs in its cytoplasmic tail whereas LILRA6 can signal through association with an activating adaptor molecule, FcRγ.

Molecular Weight: 74.6 kDa