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LILRB3 Protein (AA 24-443) (His-Avi Tag,Biotin)

LILRB3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7275190
  • Target See all LILRB3 Proteins
    LILRB3 (Leukocyte Immunoglobulin-Like Receptor B3 (LILRB3))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 24-443
    Origin
    • 14
    • 6
    Human
    Source
    • 10
    • 5
    • 4
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This LILRB3 protein is labelled with His-Avi Tag,Biotin.
    Purpose
    Biotinylated Human LILRB3/CD85a/ILT5 Protein
    Sequence
    Gly24-Glu443
    Characteristics
    Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Biotinylated Human LILRB3, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
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    Discover our top product LILRB3 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    LILRB3 (Leukocyte Immunoglobulin-Like Receptor B3 (LILRB3))
    Alternative Name
    LILRB3 (LILRB3 Products)
    Synonyms
    CD85A Protein, HL9 Protein, ILT-5 Protein, ILT5 Protein, LILRA6 Protein, LIR-3 Protein, LIR3 Protein, PIRB Protein, Gp91 Protein, Lilrb3 Protein, PIR-B Protein, Pirb Protein, NILR-1 Protein, LILRB3 Protein, leukocyte immunoglobulin like receptor B3 Protein, paired Ig-like receptor B Protein, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 Protein, LILRB3 Protein, Pirb Protein
    Background
    Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.
    Molecular Weight
    50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Tris-Bis PAGE result.
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