PSMF1 Protein (AA 1-271) (His tag)

Catalog No. ABIN666623

Product Details

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-271

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PSMF1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: PSMF1, 1-271aa , Human, His tag E.coli

Purity: > 85 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT,0.1mM PMSF

Storage: 4 °C

Target Details

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Alternative Name: PSMF1 (PSMF1 Products)

Synonyms: PI31 Protein, AW048666 Protein, BC012260 Protein, zgc:92785 Protein, proteasome inhibitor subunit 1 Protein, proteasome (prosome, macropain) inhibitor subunit 1 Protein, PSMF1 Protein, Psmf1 Protein, psmf1 Protein

Background: PSMF1 is a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Recombinant human PSMF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Proteasome inhibitor subunit 1 isoform 1, PI31. NCBI no.: NP_006805

Molecular Weight: 31.9 kDa (291aa), confirmed by MALDI-TOF

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA