CDC42 Protein (AA 1-188) (T7 tag)

Catalog No. ABIN666665

This Recombinant CDC42 protein is produced in Escherichia coli (E. coli).

Quick Overview for CDC42 Protein (AA 1-188) (T7 tag) (ABIN666665)

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-188

Purification tag / Conjugate: This CDC42 protein is labelled with T7 tag.

Sequence: MQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC

Characteristics: CDC42, 1- 188 aa, Human, T7-tagged, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol, 2 mM EDTA

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Alternative Name: CDC42

Background: Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 22.4 kDa (203aa) confirmed by MALDI-TOF

NCBI Accession: NP_001782

Pathways: MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling