Pre-mRNA Branch Site Protein p14 (SF3B14) (AA 1-125) protein (His tag)
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- Target See all Pre-mRNA Branch Site Protein p14 (SF3B14) Proteins
- Pre-mRNA Branch Site Protein p14 (SF3B14)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-125
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Characteristics
- SF3B14, 1-125aa, Human, His tag, E.coli
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product SF3B14 Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol
- Storage
- 4 °C
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- Target
- Pre-mRNA Branch Site Protein p14 (SF3B14)
- Alternative Name
- SF3B14 (SF3B14 Products)
- Synonyms
- 6030419K15Rik Protein, AV001342 Protein, Sf3b14 Protein, zgc:86708 Protein, HSPC175 Protein, Ht006 Protein, P14 Protein, SAP14 Protein, SF3B14a Protein, splicing factor 3B, subunit 6 Protein, splicing factor 3b, subunit 6 Protein, splicing factor 3b subunit 6 S homeolog Protein, splicing factor 3b subunit 6 Protein, Sf3b6 Protein, sf3b6 Protein, sf3b6.S Protein, SF3B6 Protein
- Background
- SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Splicing factor 3B, 14 kDa subunit, CGI-110, HSPC175, Ht006, P14, SAP14, SF3B14a. NCBI no.: NP_057131
- Molecular Weight
- 16.7 kDa (145aa), confirmed by MALDI-TOF
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