BLK Protein (AA 1-505) (His tag)

Catalog No. ABIN667955

This Recombinant BLK protein is produced in Baculovirus infected Insect Cells.

Quick Overview for BLK Protein (AA 1-505) (His tag) (ABIN667955)

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS)

Purity: > 80 %

Product Details

Protein Characteristics: AA 1-505

Purification tag / Conjugate: This BLK protein is labelled with His tag.

Sequence: MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ YELQP

Characteristics: BLK, 1-505aa, Human, His tag, Hi-5

Purification: > 80% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5) containing 50 % glycerol, 0.25M NaCl, 5 mM DTT, 0.1 mM PMSF, 0.1 mM EDTA

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Alternative Name: Blk

Background: BLK, also known as B lymphoid kinase, is a 55 kDa tyrosine kinase with SH3, SH2, and catalytic domains that contain consensus sequences of the Src protein tyrosine kinase family. This protein is expressed specifically in the B cell lineage and plays a role in the signaltransduction pathway that is restricted to B lymphoid cells. Recombinant human BLK protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.

Molecular Weight: 59.8 kDa (525aa)

NCBI Accession: NP_001706

Pathways: Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling