TNF alpha Protein (AA 85-223) (DYKDDDDK Tag)
Quick Overview for TNF alpha Protein (AA 85-223) (DYKDDDDK Tag) (ABIN925128)
Target
See all TNF alpha ProteinsProtein Type
Origin
Host
Application
Purity
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Protein Characteristics
- AA 85-223
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Purification tag / Conjugate
- This TNF alpha protein is labelled with DYKDDDDK Tag.
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Purpose
- Prepare a 1 mg/mL stock solution by dissolving the contents of the vial in 50 µL of sterile H20. Further dilutions should be made with medium containing 5% fetal calf serum.
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Specificity
- Recombinant soluble human TNF-alpha binds to TNF-R1. Binding to TNF-R2 requires the addition of a cross-linking anti-DYKDDDDK antibody.
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Characteristics
- The extracellular domain of human TNF-alpha (AA 85-223) is fused at the N-terminus to an 8 AA linker peptide and a DYKDDDDK tag. Exerts its biological activity in a concentration range of 0.1-1 ng/mL (WEHI 164 cells). ED50 is 0.1 ng/mL on WEHI 164 cells.
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Endotoxin Level
- <0.1 EU/µg purified protein as determined by LAL test.
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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin,His-Avi Tag)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 78-233) protein (His tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His-Avi Tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (GST tag)TNF alpha Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha Origin: Rabbit Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
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Application Notes
- Inducing Apoptosis: Recombinant human TNF-alpha protein induces apoptosis in WEHI 164 and other TNF-alpha sensitive cells. Optimal concentration varies with cell type and should be determined by testing serial dilutions on cells.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Soluble.
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Buffer
- 50 µg recombinant TNF-alpha and PBS.
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Storage
- -20 °C
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Alternative Name
- TNF alpha
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Background
- Tumor necrosis factor (TNF-alpha) is a 35 kDa type II transmembrane trimeric protein. TNF-alpha interacts with two distinct receptors, TNF-R1 and TNF-R2. Whereas both receptors activate NF- B and JNK, TNF-R1 also signals cell death. Membrane-bound TNF-alpha is efficiently cleaved and shed by a metalloprotease. The soluble form (sTNF-alpha) binds to TNF-R1. Triggering of TNF-R2-mediated signals require membrane- bound TNF-alpha. TNF-alpha is produced by many cell types including: neutrophils, activated lympho- cytes, macrophages, endothelial cells and smooth muscle cells.
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Molecular Weight
- ~19 kDa (SDS -PAGE)
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Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
Target
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