EnhancedTRAIL protein (DYKDDDDK Tag)
Origin: Human, Mouse
Host: Escherichia coli (E. coli)
Recombinant
BCA, SDS
(5 references)-
- Target
- EnhancedTRAIL
- Protein Type
- Recombinant
- Origin
- Human, Mouse
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- DYKDDDDK Tag
- Application
- Biochemical Assay (BCA), SDS-PAGE (SDS)
- Specificity
- Binds to human and mouse TRAIL receptors and human osteoprotegrin (OPG).
- Cross-Reactivity
- Human, Mouse (Murine)
- Components
- 1 Set: 2x10μg TRAIL, Soluble (human) (rec.)3x50μg TNF Ligands Enhancer (
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Source/Host: Produced in E. coli. A FLAG®-tag is fused at the N-terminus of the extracellular domain of human TRAIL (aa 95-281).Concentration: TRAIL: 0.1mg/ml after reconstitutionTNF Ligands Enhancer: 1mg/ml after reconstitutionPurity: >95% (SDS-PAGE)Formulation:Lyophilized. Contains PBS.Endotoxin Content:TRAIL: <0.01EU/μg purified protein (LAL test, Lonza).TNF Ligands Enhancer: <0.01EU/μg purified protein (LAL test, Lonza).Reconstitution:TRAIL: reconstitute with 100μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.TNF Ligands Enhancer: reconstitute each vial with 50μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
- Restrictions
- For Research Use only
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- Concentration
- Lot specific
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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: "Tumor necrosis factor-related apoptosis-inducing ligand-induced death-inducing signaling complex and its modulation by c-FLIP and PED/PEA-15 in glioma cells." in: The Journal of biological chemistry, Vol. 277, Issue 28, pp. 25020-5, (2002) (PubMed).
: "Sensitivity to TRAIL/APO-2L-mediated apoptosis in human renal cell carcinomas and its enhancement by topotecan." in: Cell death and differentiation, Vol. 7, Issue 11, pp. 1127-36, (2001) (PubMed).
: "Three adenovirus E3 proteins cooperate to evade apoptosis by tumor necrosis factor-related apoptosis-inducing ligand receptor-1 and -2." in: The Journal of biological chemistry, Vol. 276, Issue 5, pp. 3270-8, (2001) (PubMed).
: "Caspase-10 is an initiator caspase in death receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 24, pp. 13884-8, (2001) (PubMed).
: "The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines." in: FEBS letters, Vol. 503, Issue 2-3, pp. 151-7, (2001) (PubMed).
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: "Tumor necrosis factor-related apoptosis-inducing ligand-induced death-inducing signaling complex and its modulation by c-FLIP and PED/PEA-15 in glioma cells." in: The Journal of biological chemistry, Vol. 277, Issue 28, pp. 25020-5, (2002) (PubMed).
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- Target
- EnhancedTRAIL
- Background
- Induces apoptosis in a concentration range of 5-50 ng/mL in the presence of 1 μg/mL of TNF Ligands Enhancer .
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