HIV-1 Nef (full length) antibody
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- Target
- HIV-1 Nef
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Binding Specificity
- full length
- Reactivity
- Human Immunodeficiency Virus (HIV)
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Host
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Guinea Pig
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), ELISA, Dot Blot (DB), Immunoprecipitation (IP)
- Characteristics
- Whole Antiserum
- Purification
- Guinea Pig serum
- Immunogen
- Purified full-size recombinant Nef of HIV-1 subtype B expressed in E. coli
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- Application Notes
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1. Western blot: 1/1,000~1/3,000
2. Dot blot: 1/3000
3. Immunoprecipitation (assay dependent)
4. ELISA (assay dependent)
Other applications have not been tested - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Upon receipt aliquot and store at -20 C
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- Target
- HIV-1 Nef
- Target Type
- Viral Protein
- Background
- HIV-1 Nef (27 kDa) is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of cell surface receptor proteins such as CD4 and MH4 which are important for AIDS virus infection.
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