Syntaxin 12/13 (AA 1-250), (Cytoplasmic Domain) antibody

Catalog No. ABIN1742229

Product Details

Target: Syntaxin 12/13

Binding Specificity: AA 1-250, Cytoplasmic Domain

Reactivity: Rat, Chicken, Hamster, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Specificity: Specific for syntaxin 12/13.

Purification: antiserum

Immunogen: Recombinant cytoplasmic domain of rat syntaxin 13 (aa 1-250).

Application Details

Application Notes: WB: 1 : 100 up to 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC-P: 1 : 500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Brandhorst, Zwilling, Rizzoli, Lippert, Lang, Jahn: "Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 8, pp. 2701-6, (2006) (PubMed).

Bonanomi, Menegon, Miccio, Ferrari, Corradi, Kao, Benfenati, Valtorta: "Phosphorylation of synapsin I by cAMP-dependent protein kinase controls synaptic vesicle dynamics in developing neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 32, pp. 7299-308, (2005) (PubMed).

Vetrivel, Cheng, Lin, Sakurai, Li, Nukina, Wong, Xu, Thinakaran: "Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes." in: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 44945-54, (2004) (PubMed).

Bared, Buechler, Boettcher, Dayoub, Sigruener, Grandl, Rudolph, Dada, Schmitz: "Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells." in: Molecular biology of the cell, Vol. 15, Issue 12, pp. 5399-407, (2004) (PubMed).

Riedel, Antonin, Fernandez-Chacon, Alvarez de Toledo, Jo, Geppert, Valentijn, Valentijn, Jamieson, Südhof, Jahn: "Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6487-97, (2002) (PubMed).

Target Details

Target: Syntaxin 12/13