Syntaxin 12/13 (AA 1-250), (Cytoplasmic Domain) antibody

Catalog No. ABIN1742228

Product Details

Target: Syntaxin 12/13

Binding Specificity: AA 1-250, Cytoplasmic Domain

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Western Blotting (WB)

Specificity: Specific for syntaxin 12/13.

Purification: purified IgG

Immunogen: Recombinant cytoplasmic domain of rat syntaxin 13 (aa 1-250).

Clone: 151-2

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Widagdo, Chai, Ridder, Chau, Johnson, Sah, Huganir, Anggono: "Activity-Dependent Ubiquitination of GluA1 and GluA2 Regulates AMPA Receptor Intracellular Sorting and Degradation." in: Cell reports, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Deane, Ilie, Sizdahkhani, Das Gupta, Orlowski, McKinney: "Enhanced recruitment of endosomal Na+/H+ exchanger NHE6 into Dendritic spines of hippocampal pyramidal neurons during NMDA receptor-dependent long-term potentiation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 595-610, (2013) (PubMed).

Target Details

Target: Syntaxin 12/13