GalphaO 1/2 antibody

Catalog No. ABIN1742532

Product Details

Target: GalphaO 1/2

Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)

Specificity: Recognizes GalphaO 1 and GalphaO 2

Purification: purified IgG

Immunogen: Recombinant full length rodent GalphaO 2.

Clone: 101-1

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Baron, Blex, Rohrbeck, Rachakonda, Birnbaumer, Ahnert-Hilger, Brunk: "The α-subunit of the trimeric GTPase Go2 regulates axonal growth." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 782-94, (2013) (PubMed).

Winter, Brunk, Walther, Höltje, Jiang, Peter, Takamori, Jahn, Birnbaumer, Ahnert-Hilger: "Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 18, pp. 4672-80, (2005) (PubMed).

Target Details

Target: GalphaO 1/2