Interferon gamma antibody (PromoFluor 647 Premium)
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- Target See all Interferon gamma (IFNG) Antibodies
- Interferon gamma (IFNG)
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Reactivity
- Cow, Sheep, Goat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Interferon gamma antibody is conjugated to PromoFluor 647 Premium
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Application
- Flow Cytometry (FACS)
- Specificity
- Native and recombinant IFN-γ.
- Cross-Reactivity (Details)
- The monoclonal antibody cross-reacts with IFN-γ from sheep and goat.
- Purification
- Purified from in vitro cultures by protein G affinity chromatography.
- Immunogen
- Recombinant bovine IFN-gamma
- Clone
- MT307
- Isotype
- IgG2a
- Top Product
- Discover our top product IFNG Primary Antibody
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- Application Notes
- Detection of IFN-γ producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IFN-γ by flow cytometry in viable bovine PBMC. Cells were stimulated for 16 hours in the presence of PHA and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with MT307-PF647P (left panel). The specificity of MT307-PF647P was demonstrated by pre-incubating the antibody with recombinant bovine IFN-γ prior to staining of PBMC (middle panel). Matched isotype control antibody (right panel). The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- in PBS with 0.2 % BSA and 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
- Expiry Date
- 18 months
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Production of cattle lacking prion protein." in: Nature biotechnology, Vol. 25, Issue 1, pp. 132-8, (2007) (PubMed).
: "
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Production of cattle lacking prion protein." in: Nature biotechnology, Vol. 25, Issue 1, pp. 132-8, (2007) (PubMed).
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- IFNG (IFNG Products)
- Synonyms
- IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody
- Gene ID
- 281237, 443396
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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