Dynamin 1, 2, 3 (AA 2-17) antibody

Catalog No. ABIN1742248

Product Details

Target: Dynamin 1, 2, 3

Binding Specificity: AA 2-17

Reactivity: Cow, Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Recognizes dynamin 1, 2 and 3 with a preference for dynamin 1.

Purification: antiserum

Immunogen: Synthetic peptide GNRGMEDLIPLVNRLQ (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 200 up to 1 : 1000
IHC-P: 1 : 100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Rozas, Gómez-Sánchez, Tomás-Zapico, Lucas, Fernández-Chacón: "Increased neurotransmitter release at the neuromuscular junction in a mouse model of polyglutamine disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 3, pp. 1106-13, (2011) (PubMed).

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Yamada, Padilla-Parra, Park, Itoh, Chaineau, Monaldi, Cremona, Benfenati, De Camilli, Coppey-Moisan, Tramier, Galli, Takei: "Dynamic interaction of amphiphysin with N-WASP regulates actin assembly." in: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 34244-56, (2009) (PubMed).

Ferguson, Brasnjo, Hayashi, Wölfel, Collesi, Giovedi, Raimondi, Gong, Ariel, Paradise, Otoole, Flavell, Cremona, Miesenböck, Ryan, De Camilli: "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis." in: Science (New York, N.Y.), Vol. 316, Issue 5824, pp. 570-4, (2007) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details

Target: Dynamin 1, 2, 3