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5-Methylcytidine antibody

FACS, IF, IHC (fro), IHC (p), ICC Host: Mouse Monoclonal 33D3 unconjugated
Catalog No. ABIN308218
  • Target See all 5-Methylcytidine products
    5-Methylcytidine
    Host
    • 2
    • 2
    Mouse
    Clonality
    • 3
    • 1
    Monoclonal
    Conjugate
    • 4
    This 5-Methylcytidine antibody is un-conjugated
    Application
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Specificity
    Modified base 5-methylcytidine
    Purification
    Purified IgG from culture supernatant by Affinity chromatography on Protein A ceramic hyper D F - Filtration on 0.22 µm.
    Clone
    33D3
    Isotype
    IgG1
  • Application Notes
    MeDIP: Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila. Flow Cyt: Use 10ul of working dilution to label 1000000 cells in 100ul. ICC/IF: Used at a dilution of 1/200 for 16 hrs. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
    Comment

    For research use only

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/ml
    Buffer
    Phosphate Buffered Saline 10 mM – NaCl 0.15 M – pH 7.4
    Storage
    -20 °C
  • Ohta, Sakaide, Wakayama: "Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." in: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).

    Xing, Shi, Le, Lee, Silver-Morse, Li: "Evidence for transgenerational transmission of epigenetic tumor susceptibility in Drosophila." in: PLoS genetics, Vol. 3, Issue 9, pp. 1598-606, (2007) (PubMed).

  • Target
    5-Methylcytidine
    Abstract
    5-Methylcytidine Products
    Target Type
    Chemical
    Background
    DNA methylation is a DNA modification process, which is involved in the control of gene expression. The 5-Methylcytidine antibody specifically binds the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates.
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