This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This EIF2S2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-299 amino acids from the C-terminal region of human EIF2S2.
EIF2S2
Reactivity: Human, Rat, Monkey
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Rajesh, Iyer, Suragani, Ramaiah: "Intersubunit and interprotein interactions of alpha- and beta-subunits of human eIF2: Effect of phosphorylation." in: Biochemical and biophysical research communications, Vol. 374, Issue 2, pp. 336-40, (2008) (PubMed).
Sugiyama, Masuda, Shinoda, Nakamura, Tomita, Ishihama: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, (2007) (PubMed).
Mikami, Masutani, Sonenberg, Yokoyama, Imataka: "An efficient mammalian cell-free translation system supplemented with translation factors." in: Protein expression and purification, Vol. 46, Issue 2, pp. 348-57, (2006) (PubMed).
Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation.