Triglyceride Assay Kit

Catalog No. ABIN1000333

Product Details

Target: Triglyceride

Application: Biochemical Assay (BCA)

Sample Type: Serum, Plasma

Characteristics: Sensitive and accurate. Use as little as 10 L samples. Linear detection range 0.01 mmol/L to 1.0 mmol/L (0.88 mg/dL to 88.5 mg/dL) triglyceride.
Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.
Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Components: Assay Buffer: 24 mL. ATP: 250 L. Dye Reagent: 220 L. Enzyme Mix: 500 L. Lipase: 1000 L. Standard: 100 L (equivalent to 100 mmol/L Triglyceride).

Material not included: Pipeting devices, centrifuge tubes, clear flat bottom 96-well plates (e.g. Corning Costar) and plate reader.

Application Details

Application Notes: Direct Assays: triglyceride in biological samples (e.g. serum and plasma).
Drug Discovery/Pharmacology: effects of drugs on triglyceride metabolism.

Calculation of Results:

Subtract ODH2O (water, #4) from the standard OD values and plot the OD against standard concentrations.
Conversions: 1 mmol/L triglyceride equals 88.5 mg/dL or 10 ppm.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References

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Bytautiene, Tamayo, Kechichian, Drever, Gamble, Hankins, Saade: "Prepregnancy obesity and sFlt1-induced preeclampsia in mice: developmental programming model of metabolic syndrome." in: American journal of obstetrics and gynecology, Vol. 204, Issue 5, pp. 398.e1-8, (2011) (PubMed).

Orban, Palczewska, Palczewski: "Retinyl ester storage particles (retinosomes) from the retinal pigmented epithelium resemble lipid droplets in other tissues." in: The Journal of biological chemistry, Vol. 286, Issue 19, pp. 17248-58, (2011) (PubMed).

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Oh, Kim, Jang, Byeon, Ryu, Kim, Ha: "Semipurified fractions from the submerged-culture broth of Agaricus blazei Murill reduce blood glucose levels in streptozotocin-induced diabetic rats." in: Journal of agricultural and food chemistry, Vol. 58, Issue 7, pp. 4113-9, (2010) (PubMed).

Tucci, Primassin, Ter Veld, Spiekerkoetter: "Medium-chain triglycerides impair lipid metabolism and induce hepatic steatosis in very long-chain acyl-CoA dehydrogenase (VLCAD)-deficient mice." in: Molecular genetics and metabolism, Vol. 101, Issue 1, pp. 40-7, (2010) (PubMed).

Tam, Vemuri, Liu, Bátkai, Mukhopadhyay, Godlewski, Osei-Hyiaman, Ohnuma, Ambudkar, Pickel, Makriyannis, Kunos: "Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2953-66, (2010) (PubMed).

Kim, Xiao, Wang, Lahusen, Xu, Vassilopoulos, Vazquez-Ortiz, Jeong, Park, Ki, Gao, Deng: "Hepatic-specific disruption of SIRT6 in mice results in fatty liver formation due to enhanced glycolysis and triglyceride synthesis." in: Cell metabolism, Vol. 12, Issue 3, pp. 224-36, (2010) (PubMed).

Lee, Kim, Kim, Shin, Baik: "GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats." in: Lipids, Vol. 43, Issue 5, pp. 419-29, (2008) (PubMed).

Target Details

Target: Triglyceride

Abstract: Triglyceride Products

Background: Quantitative determination of triglyceride by colorimetric (570nm) method.
Procedure: 30 min.

Triglyceride, also known as Triacyltriglyceride or Triacyl-Glyceride, is the main constituent in vegetable oil and animal fats. Triglycerides play an important role as energy sources and transporters of dietary fat. In the human body, high levels of triglycerides in the bloodstream have been linked to atherosclerosis, heart disease and pancreatitis. Simple, direct and automation-ready procedures for measuring triglyceride concentrations find wide applications in research and drug discovery. This triglyceride assay uses a single Working Reagent that combines triglyceride hydrolysis and glycerol determination in one step, in which a dye reagent is oxidized to form a colored product. The color intensity at 570nm is directly proportional to triglyceride concentration in the sample.