Homer 1/2/3 (N-Term) antibody

Catalog No. ABIN1742340

Product Details

Target: Homer 1/2/3

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Western Blotting (WB)

Specificity: The first 120 aa were used for affinity purification. According to Soloviev et al. (2000), they are homologous in Homer 1 a-d, Homer 2 a-d, and Homer 3 a-d.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Purified recombinant N-terminal parts of Homer 1, 2 and 3.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet
ELISA: not tested yet

Comment:

ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Tao-Cheng, Thein, Yang, Reese, Gallant: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).

Tronson, Guzman, Guedea, Huh, Gao, Schwarz, Radulovic: "Metabotropic glutamate receptor 5/Homer interactions underlie stress effects on fear." in: Biological psychiatry, Vol. 68, Issue 11, pp. 1007-15, (2010) (PubMed).

Target Details

Target: Homer 1/2/3

Background: Synonyms: VesL 1, Syn47