Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
B
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This POLR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the C-terminal region of human POLR2C.
POLR2C
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IF: 1:25. WB: 1:1000. IHC-P: 1:100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Dammann, Pfeifer: "Cloning and characterization of the human RNA polymerase I subunit hRPA40." in: Biochimica et biophysica acta, Vol. 1396, Issue 2, pp. 153-7, (1998) (PubMed).
Pati, Weissman: "The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes." in: The Journal of biological chemistry, Vol. 265, Issue 15, pp. 8400-3, (1990) (PubMed).
Target
POLR2C
(Polymerase (RNA) II (DNA Directed) Polypeptide C, 33kDa (POLR2C))
RPB3 antibody, RPB31 antibody, hRPB33 antibody, hsRPB3 antibody, 33kDa antibody, Rpo2-3 antibody, mRBP31 antibody, zgc:56285 antibody, wu:fk48d01 antibody, MGC76329 antibody, MGC81245 antibody, POLR2C antibody, polr2c antibody, rpb3 antibody, RNA polymerase II subunit C antibody, polymerase (RNA) II (DNA directed) polypeptide C antibody, polymerase (RNA) II (DNA directed) polypeptide C, 33kDa antibody, polymerase (RNA) II (DNA directed) polypeptide C, 33kDa S homeolog antibody, POLR2C antibody, Polr2c antibody, polr2c antibody, polr2c.S antibody
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).