5-Carboxylcytosine antibody

Catalog No. ABIN2668976

Product Details

Target: 5-Carboxylcytosine

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purification: None

Immunogen: This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References

Eleftheriou, Pascual, Wheldon, Perry, Abakir, Arora, Johnson, Auer, Ellis, Madhusudan, Ruzov: "5-Carboxylcytosine levels are elevated in human breast cancers and gliomas." in: Clinical epigenetics, Vol. 7, pp. 88, (2015) (PubMed).

Nettersheim, Heukamp, Fronhoffs, Grewe, Haas, Waha, Honecker, Waha, Kristiansen, Schorle: "Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development." in: PLoS ONE, Vol. 8, Issue 12, pp. e82881, (2014) (PubMed).

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." in: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Zhang, Szulwach, Hon, Song, Park, Yu, Lu, Dai, Wang, Street, Tan, Min, Ren, Jin, He: "Tet-mediated covalent labelling of 5-methylcytosine for its genome-wide detection and sequencing." in: Nature communications, Vol. 4, pp. 1517, (2013) (PubMed).

Inoue, Shen, Dai, He, Zhang: "Generation and replication-dependent dilution of 5fC and 5caC during mouse preimplantation development." in: Cell research, Vol. 21, Issue 12, pp. 1670-6, (2011) (PubMed).

Target Details

Target: 5-Carboxylcytosine

Target Type: Chemical

Gene ID: 57279