Glucocorticoid Receptor antibody (N-Term)
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- Target See all Glucocorticoid Receptor (NR3C1) Antibodies
- Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Glucocorticoid Receptor antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Cross-Reactivity
- Human, Mouse, Rat
- Characteristics
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Rabbit Polyclonal antibody to Glucocorticoid receptor (nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor))
Glucocorticoid receptor antibody - Purification
- Purified by antigen-affinity chromatography.
- Grade
- KO Validated
- Immunogen
- Recombinant protein encompassing a sequence within the N-terminus region of human Glucocorticoid Receptor. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product NR3C1 Primary Antibody
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- Application Notes
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: A431 , mouse heart , rat heart , HeLa
Validation: KO/KD
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-α protein levels and activity." in: Oncogene, Vol. 34, Issue 2, pp. 154-64, (2015) (PubMed).
: "Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses." in: Nucleic acids research, Vol. 41, Issue 7, pp. 4036-48, (2013) (PubMed).
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Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-α protein levels and activity." in: Oncogene, Vol. 34, Issue 2, pp. 154-64, (2015) (PubMed).
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- Target
- Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
- Alternative Name
- nuclear receptor subfamily 3 group C member 1 (NR3C1 Products)
- Synonyms
- GR antibody, gcr antibody, grl antibody, xGR antibody, gccr antibody, gr antibody, zgc:113038 antibody, NR3C1 antibody, GCL antibody, LOC100302444 antibody, Gcr antibody, Grl antibody, Grl-1 antibody, Grl1 antibody, GRL antibody, GCCR antibody, GCR antibody, NO antibody, GR-A antibody, nr3c1 antibody, nuclear receptor subfamily 3 group C member 1 antibody, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody, nuclear receptor subfamily 3, group C, member 1 antibody, glucocorticoid receptor antibody, NR3C1 antibody, nr3c1 antibody, LOC100302444 antibody, Nr3c1 antibody
- Background
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The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.
Cellular Localization: Cytoplasm , Nucleus - Molecular Weight
- 86 kDa
- Gene ID
- 2908
- UniProt
- P04150
- Pathways
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process
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