PsbO antibody

Catalog No. ABIN2559392

Product Details

Target: PsbO

Reactivity: Spinach, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: dicots including: Nicotiana tabacum, Solanum lycopersicum, trees: Populus trichocarpa

Characteristics: Expected / apparent Molecular Weight of the Antigene: 35 / 33 kDa

Purification: affinity purified

Immunogen: native purified 33 kDa protein from Spinacia oleracea UniProt P12359

Application Details

Application Notes: Recommended Dilution 1 : 2000 - 1 : 5000 with standard ECL (WB).

Comment:

This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References

Lin, Liu, Kuo, Tsai, Feng, Huang, Chien: "Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT." in: Bioresource technology, Vol. 143, pp. 154-62, (2013) (PubMed).

Target Details

Target: PsbO

Background: PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Molecular Weight: expected: 35 kDa, apparent: 33 kDa