PsbO antibody

Details for Product No. ABIN93573
Antigen
Reactivity
Arabidopsis thaliana, Barley (Hordeum vulgare), Tobacco (Nicotiana tabacum)
4
1
1
1
1
Host
Rabbit
5
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
4
1
Options
Immunogen N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 At5g66570 and 2 proteins At5g50820
Cross-Reactivity (Details) No cross-reactivity with: Chlamydomonas reinhardtii, Synechococcus
Characteristics Expected / apparent Molecular Weight of the Antigene: 33 kDa
Purification serum
Antigen
Background AGI Code: At5g66570
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.
Molecular Weight 33 kDa
UniProt Q9S841, P23321
Application Notes Recommended Dilution: 1 : 1000 with standard ECL (WB).
Loading based on 50 - 100 ng of chlorophyll is enough to obtain good signal withthis antibody
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 µL, of sterile water.
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Supplier Images
Image no. 1 for anti-PsbO antibody (ABIN93573) From left to right: Arabidopsis thaliana (1), Horderum vulgare (2), Chlamydmononas re...
Product cited in: Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, 2012 (PubMed).

Lundin, Nurmi, Rojas-Stuetz, Aro, Adamska, Spetea: "Towards understanding the functional difference between the two PsbO isoforms in Arabidopsis thaliana--insights from phenotypic analyses of psbo knockout mutants." in: Photosynthesis research, Vol. 98, Issue 1-3, pp. 405-14, 2008 (PubMed).