PsbO antibody
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- Target
- PsbO
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Reactivity
- Arabidopsis thaliana, Barley, Nicotiana tabacum
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
- Cross-Reactivity (Details)
- No cross-reactivity with: Chlamydomonas reinhardtii, Synechococcus
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 33 kDa
- Purification
- serum
- Immunogen
- N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 At5g66570 and 2 proteins At5g50820
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- Application Notes
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Recommended Dilution: 1 : 1000 with standard ECL (WB).
Loading based on 50 - 100 ng of chlorophyll is enough to obtain good signal withthis antibody - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL, of sterile water.
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
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LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).
: "Towards understanding the functional difference between the two PsbO isoforms in Arabidopsis thaliana--insights from phenotypic analyses of psbo knockout mutants." in: Photosynthesis research, Vol. 98, Issue 1-3, pp. 405-14, (2008) (PubMed).
: "
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LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).
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- Target
- PsbO
- Background
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AGI Code: At5g66570
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2. - Molecular Weight
- 33 kDa
- UniProt
- Q9S841, P23321
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