PsbO antibody

Catalog No. ABIN7676202

Cited in 2+ publications. The Rabbit Polyclonal anti-PsbO antibody (ABIN7676202) specifically detects PsbO in WB, IP and IHC. The antibody is reactive with Arabidopsis thaliana, Barley, Cucumis sativus, Manihot esculenta, Nicotiana tabacum, Pea, Sinapis alba, Triticum aestivum and Zea mays samples.

Quick Overview for PsbO antibody (ABIN7676202)

Target: PsbO

Reactivity: Arabidopsis thaliana, Barley, Cucumis sativus, Manihot esculenta, Nicotiana tabacum, Pea, Sinapis alba, Triticum aestivum, Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Product Details

Purpose: Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)

Cross-Reactivity (Details): Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Manihot esculenta, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Triticum aestivum, Zea mays
Not reactive in: Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Predicted Reactivity: Brassica oleracea,Pisum sativum,Populus tremula,Picea sitcHensis,Vitis vinifera

Purification: Serum

Immunogen: N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 and PsbO2 isoforms. UniProt: P23321

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

Lundin, Nurmi, Rojas-Stuetz, Aro, Adamska, Spetea: "Towards understanding the functional difference between the two PsbO isoforms in Arabidopsis thaliana--insights from phenotypic analyses of psbo knockout mutants." in: Photosynthesis research, Vol. 98, Issue 1-3, pp. 405-14, (2008) (PubMed).

Target Details

Target: PsbO

Background:

Background: The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Additional information: Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody

Molecular Weight: 33 kDa

Gene ID: 836789

UniProt: P23321, Q9S841