Keratin Hair Cortex antibody

Catalog No. ABIN108614

Product Details

Target: Keratin Hair Cortex

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Specificity: This antibody is specific for the family of hair cortex keratins

Purification: Protein G purified

Immunogen: Human hair keratins

Clone: AE13

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as guideline try, WB: 1/1000 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 - 46 kDa). Flow Cyt: 1/20 - 1/50

Comment:

Myeloma, fusion partners: P3X63Ag. 8.653 myeloma cells,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody in PBS (PH 7.2) 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References

Chen, Jarrell, Guo, Lang, Atit: "Dermal β-catenin activity in response to epidermal Wnt ligands is required for fibroblast proliferation and hair follicle initiation." in: Development (Cambridge, England), Vol. 139, Issue 8, pp. 1522-33, (2012) (PubMed).

Larouche, Cuffley, Paquet, Germain: "Tissue-engineered skin preserving the potential of epithelial cells to differentiate into hair after grafting." in: Tissue engineering. Part A, Vol. 17, Issue 5-6, pp. 819-30, (2011) (PubMed).

Youssef, Van Keymeulen, Lapouge, Beck, Michaux, Achouri, Sotiropoulou, Blanpain: "Identification of the cell lineage at the origin of basal cell carcinoma." in: Nature cell biology, Vol. 12, Issue 3, pp. 299-305, (2010) (PubMed).

Van Keymeulen, Mascre, Youseff, Harel, Michaux, De Geest, Szpalski, Achouri, Bloch, Hassan, Blanpain: "Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis." in: The Journal of cell biology, Vol. 187, Issue 1, pp. 91-100, (2009) (PubMed).

Suzuki, Yamaguchi, Villacorte, Mihara, Akiyama, Shimizu, Taketo, Nakagata, Tsukiyama, Yamaguchi, Birchmeier, Kato, Yamada: "Embryonic hair follicle fate change by augmented beta-catenin through Shh and Bmp signaling." in: Development (Cambridge, England), Vol. 136, Issue 3, pp. 367-72, (2009) (PubMed).

List, Szabo, Molinolo, Nielsen, Bugge: "Delineation of matriptase protein expression by enzymatic gene trapping suggests diverging roles in barrier function, hair formation, and squamous cell carcinogenesis." in: The American journal of pathology, Vol. 168, Issue 5, pp. 1513-25, (2006) (PubMed).

Kajikawa, Baba, Tomaru, Watanabe, Koganei, Tsuji-Kawahara, Matsumoto, Yamamoto, Miyazawa, Maenaka, Ishizu, Kasahara: "MHC class I-like MILL molecules are beta2-microglobulin-associated, GPI-anchored glycoproteins that do not require TAP for cell surface expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 5, pp. 3108-15, (2006) (PubMed).

Dhouailly, Xu, Manabe, Schermer, Sun et al.: "Expression of hair-related keratins in a soft epithelium: subpopulations of human and mouse dorsal tongue keratinocytes express keratin markers for hair-, skin- and esophageal-types of ..." in: Experimental cell research, Vol. 181, Issue 1, pp. 141-58, (1989) (PubMed).

Target Details

Target: Keratin Hair Cortex

Alternative Name: Hair Cortex Cytokeratin

Background: Synonyms:
CK-40 antibody,Cytokeratin-40 antibody,Keratin, type I cytoskeletal 40antibody,Keratin-40 antibody,KRT40 antibody,Type I hair keratin Ka36antibody