GABRA3 antibody (AA 29-43)
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- Target See all GABRA3 Antibodies
- GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))
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Binding Specificity
- AA 29-43
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Reactivity
- Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GABRA3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificity
- Specific for GABA-A receptor alpha3.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Synthetic peptide (aa 29-43 of rat GABA-A receptor alpha3 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
- Top Product
- Discover our top product GABRA3 Primary Antibody
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- Application Notes
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 - Comment
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WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala." in: PLoS ONE, Vol. 9, Issue 4, pp. e94666, (2014) (PubMed).
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Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala." in: PLoS ONE, Vol. 9, Issue 4, pp. e94666, (2014) (PubMed).
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- Target
- GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))
- Alternative Name
- GABA-A Receptor Alpha3 (GABRA3 Products)
- Synonyms
- GABRA3 antibody, Gabra-3 antibody, si:ch211-113k9.1 antibody, gamma-aminobutyric acid type A receptor alpha3 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 3 antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 3 antibody, gamma-aminobutyric acid receptor subunit alpha-3 antibody, GABRA3 antibody, gabra3 antibody, Gabra3 antibody, LOC100653496 antibody
- Background
- Synonyms: GABA(A) R alpha3
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