T4 SSB (Active) Protein
Origin: Bacteriophage T4
Host: Escherichia coli (E. coli)
Recombinant
> 95 % of protein determined by SDS-PAGE (CBB staining)
Func
Active
(2 references)-
- Target
- T4 SSB
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Bacteriophage T4
- Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func)
- Characteristics
- The absence of endonucleases and exonucleases was confirmed.
- Purity
- > 95 % of protein determined by SDS-PAGE (CBB staining)
-
- Application Notes
-
1. Promoting DNA replication and recombination by stabilizing single-stranded DNA
2. Increase specificity and yields of long PCR products - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 10 mg/mL
- Buffer
- 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
-
-
: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).
: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." in: Nature, Vol. 227, Issue 5265, pp. 1313-8, (1970) (PubMed).
-
: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).
-
- Target
- T4 SSB
- Target Type
- Phage Protein
- Background
- T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.
- UniProt
- P03695
-
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