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to understand actin-mediated change in protein localization during acrosome reaction, distribution of CAPZA3 was analyzed; like IZUMO1 (show IZUMO1 Proteins), CAPZA3 shows dynamic pattern of localization; movements follow different temporal pattern than changes seen with IZUMO1 (show IZUMO1 Proteins)
These observations strongly suggest the missense mutation in Capza3 is responsible for the mutant phenotype of repro32/repro32 sperm and regulation of F-actin dynamics
This gene encodes an actin capping protein, one of the F-actin capping protein alpha subunit family. The encoded protein is predominantly localized to the neck region of ejaculated sperm, other immunohistochemical signals were found in the tail and postacrosomal regions. The encoded protein may also form heterodimers of alpha and beta subunits. This protein may be important in determining sperm architecture and male fertility.
capping protein (actin filament) muscle Z-line, alpha 3
, capping protein alpha 3
, f-actin-capping protein subunit alpha-3-like
, CapZ alpha-3
, F-actin capping protein alpha-3 subunit
, F-actin-capping protein subunit alpha-3
, germ cell-specific protein 3
, capZ alpha-3
, cp alpha3