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Human Amphiregulin ELISA Kit

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Antigen
Amphiregulin (AREG) ELISA Kits
  • AR
  • AREG
  • AREG/SDGF
  • AREGB
  • CRDGF
  • SDGF
  • Sdgf
Epitope
AA 101-198
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
21
19
14
7
7
6
6
5
5
3
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN1889290
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414351 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 6
0.32693866 ABIN1113368 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32693866 ABIN2708069 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN365786 Sandwich ELISA Serum, Plasma 4.7-300 pg/mL 4.7 pg/mL Log in to see
0.0012117105 ABIN3032886 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 5.5-4000 pg/mL 5.5 pg/mL Log in to see
0.0012117105 ABIN624943 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 6
0.0012117105 ABIN511226 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
0.0012117105 ABIN1115707 Sandwich ELISA Serum, Plasma, Biological Fluids Log in to see
0.0012117105 ABIN1568493 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN2683490 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see 1
0.0012117105 ABIN2683489 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Milk, Saliva, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2541214 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012117105 ABIN2535806 Tissue Homogenate, Cell Lysate, Biological Fluids 1-70 pg/mL 1 pg/mL Log in to see
0.0012117105 ABIN455651 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2055252 Sandwich ELISA Log in to see
0.0012117105 ABIN2089513 Sandwich ELISA Log in to see
0.0012117105 ABIN2115923 Log in to see

Top referenced Amphiregulin ELISA Kit for ELISA

Similar Amphiregulin ELISA Kits

Application / Reactivity Human
ELISA 18 ELISA Kits

General

Antigen Amphiregulin (AREG) ELISA Kits
Epitope AA 101-198
Reactivity Human
Kits with alternative reactivity to:
(21), (19), (14), (7), (7), (6), (6), (5), (5), (3), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details Amphiregulin ELISA Kit

Target details Application Details Handling References for Amphiregulin Kit (ABIN1889290) Images
Purpose For quantitative detection of human Amphiregulin in cell culture supernates, serum, plasma(heparin, EDTA), saliva and human milk.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Milk
Detection Method Colorimetric
Specificity Natural and recombinant human Amphiregulin
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human Amphiregulin antibod - 1
  • Lyophilized recombinant human Amphiregulin standar - 10ng/tubex2
  • Biotinylated anti- human Amphiregulin antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: S101-K198

Target details

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Antigen
Alternative Name AREG (AREG ELISA Kit Abstract)
Background Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.
Gene ID 374
UniProt P15514
Pathways RTK Signaling, EGFR Signaling Pathway

Application Details

Product Details Amphiregulin ELISA Kit Target details Handling References for Amphiregulin Kit (ABIN1889290) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Amphiregulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Amphiregulin has been precoated onto 96-well plates. Standards(E.coli, S101-K198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Amphiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Amphiregulin amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Amphiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Amphiregulin standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 115, Standard deviation: 6.67, CV(%): 5.8
  • Sample 2: n=16, Mean(pg/ml): 351, Standard deviation: 15.1, CV(%): 4.3
  • Sample 3: n=16, Mean(pg/ml): 633, Standard deviation: 29.75, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 104, Standard deviation: 8.94, CV(%): 8.6
  • Sample 2: n=24, Mean(pg/ml): 339, Standard deviation: 24.75, CV(%): 7.3
  • Sample 3: n=24, Mean(pg/ml): 620, Standard deviation: 32.24, CV(%): 5.2
Restrictions For Research Use only

Handling

Product Details Amphiregulin ELISA Kit Target details Application Details References for Amphiregulin Kit (ABIN1889290) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Amphiregulin Kit (ABIN1889290)

Product Details Amphiregulin ELISA Kit Target details Application Details Handling Images back to top
Background publications

Ciarloni, Mallepell, Brisken: "Amphiregulin is an essential mediator of estrogen receptor alpha function in mammary gland development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 13, pp. 5455-60, 2007

Zaiss, Yang, Shah, Kobie, Urban, Mosmann: "Amphiregulin, a TH2 cytokine enhancing resistance to nematodes." in: Science (New York, N.Y.), Vol. 314, Issue 5806, pp. 1746, 2006

Shoyab, Plowman, McDonald, Bradley, Todaro: "Structure and function of human amphiregulin: a member of the epidermal growth factor family." in: Science (New York, N.Y.), Vol. 243, Issue 4894 Pt 1, pp. 1074-6, 1989

Images

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Supplier Images
ELISA image for Amphiregulin ELISA Kit (AREG) (ABIN1889290) Human Amphiregulin(AR) PicoKine ELISA Kit standard curve