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Mouse (Murine) Angiopoietin 1 ELISA Kit

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Antigen
Angiopoietin 1 (ANGPT1) ELISA Kits
  • 1110046O21Rik
  • AGP1
  • AGPT
  • Agpt
  • Agpt1
  • Ang-1
  • ANG-1
  • ANG1
  • Ang1
  • ang1
  • angpt1
  • DHCR14A
  • MGC80788
  • NET47
  • wu:fc76g06
Epitope
AA 16-497
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
27
14
13
9
8
7
4
4
3
3
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10.000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1889436
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3165579 ABIN415585 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see 2
0.3165579 ABIN1113417 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.16 ng/mL Log in to see
0.3165579 ABIN1568356 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012210747 ABIN365440 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 0.312-20 pg/mL 0.312 pg/mL Log in to see
0.0012210747 ABIN627488 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 1-25 ng/mL 1 ng/mL Log in to see
0.0012210747 ABIN511907 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012210747 ABIN454256 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 0.78-50 ng/mL 0.78 ng/mL Log in to see
0.0012210747 ABIN2748759 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.0012210747 ABIN2683441 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
0.0012210747 ABIN2541287 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012210747 ABIN2945230 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012210747 ABIN2055896 Sandwich ELISA Log in to see
0.0012210747 ABIN2112079 Competition ELISA Log in to see

Top referenced Angiopoietin 1 ELISA Kit for ELISA

Similar Angiopoietin 1 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 14 ELISA Kits

General

Antigen Angiopoietin 1 (ANGPT1) ELISA Kits
Epitope AA 16-497
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(27), (14), (13), (9), (8), (7), (4), (4), (3), (3), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10.000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details Angiopoietin 1 ELISA Kit

Target details Application Details Handling References for Angiopoietin 1 Kit (ABIN1889436) Images
Purpose For quantitative detection of mouse Angiopoietin-1 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse Angiopoietin-1
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse Angiopoietin-1 antibod - 1
  • Lyophilized recombinant mouse Angiopoietin-1 standar - 10ng/tubex2
  • Biotinylated anti- mouse Angiopoietin-1 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: H16-F497

Target details

Product Details Angiopoietin 1 ELISA Kit Application Details Handling References for Angiopoietin 1 Kit (ABIN1889436) Images back to top
Antigen
Alternative Name ANGPT1 (ANGPT1 ELISA Kit Abstract)
Background Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
Gene ID 11600
UniProt O08538
Pathways RTK Signaling

Application Details

Product Details Angiopoietin 1 ELISA Kit Target details Handling References for Angiopoietin 1 Kit (ABIN1889436) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse Angiopoietin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Angiopoietin-1 has been precoated onto 96-well plates. Standards(NSO, H16-F497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Angiopoietin-1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Angiopoietin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Angiopoietin-1 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.28, Standard deviation: 0.055, CV(%): 4.3
  • Sample 2: n=16, Mean(ng/ml): 3.54, Standard deviation: 0.131, CV(%): 3.7
  • Sample 3: n=16, Mean(ng/ml): 6.85, Standard deviation: 0.301, CV(%): 4.4,
  • Sample 1: n=24, Mean(ng/ml): 1.59, Standard deviation: 0.086, CV(%): 5.4
  • Sample 2: n=24, Mean(ng/ml): 3.83, Standard deviation: 0.181, CV(%): 4.7
  • Sample 3: n=24, Mean(ng/ml): 6.97, Standard deviation: 0.369, CV(%): 5.3
Restrictions For Research Use only

Handling

Product Details Angiopoietin 1 ELISA Kit Target details Application Details References for Angiopoietin 1 Kit (ABIN1889436) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Angiopoietin 1 Kit (ABIN1889436)

Product Details Angiopoietin 1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Ward, Grosios, Markham, Jones: "Genomic structures of the human angiopoietins show polymorphism in angiopoietin-2." in: Cytogenetics and cell genetics, Vol. 94, Issue 3-4, pp. 147-54, 2002

Images

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Supplier Images
ELISA image for Angiopoietin 1 ELISA Kit (ANGPT1) (ABIN1889436) Mouse Angiopoietin-1 PicoKine ELISA Kit standard curve