Rat (Rattus) Angiopoietin 2 ELISA Kit

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Antigen
Angiopoietin 2 (ANGPT2) ELISA Kits
  • ang2
  • angpt2
  • ANG-2
  • ANGPT2
  • Agpt2
  • Ang-2
  • Ang2
  • AGPT2
  • ANG2
  • ANGIOPOIETIN-2
  • angiopoietin 2b
  • angiopoietin-2
  • angiopoietin 2
  • angpt2b
  • ANG-2
  • ANGPT2
  • LOC100225992
  • Angpt2
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
28
16
13
11
8
3
3
3
2
2
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
0.187-12 ng/mL
Minimum Detection Limit
0.187 ng/mL
Application
ELISA
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Catalog No. ABIN1568364
$ 891.06
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.865132 ABIN416301 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 12.50-800 pg/mL 12.50 pg/mL Log in to see
9.865132 ABIN365550 Sandwich ELISA Serum, Plasma, Tissue Homogenate 47-3000 pg/mL 47 pg/mL Log in to see
9.865132 ABIN1113386 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN626658 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
1 ABIN5591640 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5519862 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN579275 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN2541292 Serum, Plasma, Biological Fluids 2.8-150 pg/mL 2.8 pg/mL Log in to see
1 ABIN4967111 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.187-12 ng/mL 0.187 ng/mL Log in to see
1 ABIN2541297 Sandwich ELISA Serum, Plasma, Biological Fluids 12.5-800 pg/mL 12.5 pg/mL Log in to see
1 ABIN5510731 Sandwich ELISA Log in to see
1 ABIN2945237 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5072649 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

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General

Antigen Angiopoietin 2 (ANGPT2) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(28), (16), (13), (11), (8), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.187-12 ng/mL
Minimum Detection Limit 0.187 ng/mL
Application ELISA
Supplier Log in to see

Product Details Angiopoietin 2 ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ANGPT2 in rat serum, plasma and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.09 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Target details

Product Details Angiopoietin 2 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name ANGPT2 (ANGPT2 ELISA Kit Abstract)
Background Synonyms: Agpt2,ANG-2,Angiopoietin-2,Angpt2
Gene ID 89805
UniProt O35462
Pathways RTK Signaling

Application Details

Product Details Angiopoietin 2 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Assay Procedure The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Handling

Product Details Angiopoietin 2 ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months

Images

Product Details Angiopoietin 2 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Angiopoietin 2 ELISA Kit (ANGPT2) (ABIN1568364) Angiopoietin 2 (ANGPT2) ELISA Kit