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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)||Hamster|
|Western Blotting (WB)||15 Antibodies||8 Antibodies||10 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies||3 Antibodies||3 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies||11 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Antigen||Actin Related Protein 2/3 Complex, Subunit 5, 16kDa (ARPC5) Antibodies|
|Epitope||AA 19-24 Alternatives|
|Reactivity||Human, Rat (Rattus), Mouse (Murine), Hamster Alternatives|
|Conjugate||This ARPC5 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-ARPC5 AntibodyTarget Details ARPC5 Application Details Handling References for anti-ARPC5 Antibody (ABIN1742556) Images
|Specificity||Specific for p16-Arc.|
|Purification||purified IgG. Azide was added before lyophilization.|
|Immunogen||Recombinant full length p16-Arc. Epitope YDENKF (aa 19-24 in human p16-Arc).|
Target Details ARPC5Product Details anti-ARPC5 Antibody Application Details Handling References for anti-ARPC5 Antibody (ABIN1742556) Images back to top
|Alternative Name||p16-Arc (ARPC5 Antibody Abstract)|
|Background||Synonyms: Arp C5|
Application DetailsProduct Details anti-ARPC5 Antibody Target Details ARPC5 Handling References for anti-ARPC5 Antibody (ABIN1742556) Images back to top
WB: 1 : 100 up to 1 : 2000 (AP staining)
IP: not tested yet
IHC: not tested yet
|Restrictions||For Research Use only|
HandlingProduct Details anti-ARPC5 Antibody Target Details ARPC5 Application Details References for anti-ARPC5 Antibody (ABIN1742556) Images back to top
|Reconstitution||For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.|
|Buffer||PBS, 0.02% sodium azide|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/ml protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.|
|Storage Comment||Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.|
References for anti-ARPC5 Antibody (ABIN1742556)Product Details anti-ARPC5 Antibody Target Details ARPC5 Application Details Handling Images back to top
|Product cited in:||
Oelkers, Vinzenz, Nemethova et al.: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, 2011 (PubMed).
Lladó, Timpson, Vilà de Muga et al.: "Protein kinase Cdelta and calmodulin regulate epidermal growth factor receptor recycling from early endosomes through Arp2/3 complex and cortactin." in: Molecular biology of the cell, Vol. 19, Issue 1, pp. 17-29, 2008 (PubMed).
Steffen, Faix, Resch et al.: "Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes." in: Molecular biology of the cell, Vol. 17, Issue 6, pp. 2581-91, 2006 (PubMed).
Takamori, Holt, Stenius et al.: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, 2006 (PubMed).
ImagesProduct Details anti-ARPC5 Antibody Target Details ARPC5 Application Details Handling References for anti-ARPC5 Antibody (ABIN1742556) back to top