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Human BDNF ELISA Kit

Recommended BDNF Product (supplied by: Log in to see )

Antigen
Brain-Derived Neurotrophic Factor (BDNF) ELISA Kits
  • ANON2
  • bdnf
  • Bdnf
  • BDNF
  • bdnf-A
  • BULN2
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
34
24
22
11
8
8
7
6
6
5
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
93.8-6000 pg/mL
Minimum Detection Limit
93.8 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2506943
$ 433.40
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN411257 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate) 31.2-2000 pg/mL 31.2 pg/mL Log in to see 7
0.32693866 ABIN414355 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see 1
0.32693866 ABIN924875 Sandwich ELISA Cell Lysate, Serum, Plasma 47-1500 pg/mL 47 pg/mL Log in to see
0.32693866 ABIN1113812 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.32693866 ABIN1888473 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 80-16 ng/mL 80 ng/mL Log in to see
0.32693866 ABIN454600 Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.32693866 ABIN2708078 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32693866 ABIN2708077 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.32693866 ABIN2113169 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Log in to see
0.0012117105 ABIN365224 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 0.3125-20 ng/mL 0.3125 ng/mL Log in to see 2
0.0012117105 ABIN1995054 Sandwich ELISA Serum, Plasma, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
0.0012117105 ABIN624945 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 35
0.0012117105 ABIN3198503 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012117105 ABIN510366 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012117105 ABIN2683533 Sandwich ELISA Plasma (citrate), Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see 3
0.0012117105 ABIN1112560 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2747979 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 12-3000 pg/mL Log in to see
0.0012117105 ABIN511247 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012117105 ABIN2042356 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2113168 Cell Lysate, Serum, Plasma 47-1500 pg/mL 47 pg/mL Log in to see

Top referenced BDNF ELISA Kit for ELISA

  • Human BDNF ELISA Kit for Sandwich ELISA - ABIN624945 (35 Publications): Liu, Jiao, Wang, Bu, Yao, Xiang, Wang, Wang, Deng, Li, Zhou, Zhou, Wang: Associations Between ApoE?4 Carrier Status and Serum BDNF Levels--New Insights into the Molecular Mechanism of ApoE?4 Actions in Alzheimer's Disease. in Molecular neurobiology 2015 (PubMed)

  • Human BDNF ELISA Kit for Sandwich ELISA - ABIN411257 (7 Publications): Shi, You, Yuan, Zhang, Li, Hou: Plasma BDNF and tPA are associated with late-onset geriatric depression. in Psychiatry and clinical neurosciences 2010 (PubMed)

  • Human BDNF ELISA Kit for Sandwich ELISA - ABIN2683533 (3 Publications): Lyons, Mamounas, Ricaurte, Coppola, Reid, Bora, Wihler, Koliatsos, Tessarollo: Brain-derived neurotrophic factor-deficient mice develop aggressiveness and hyperphagia in conjunction with brain serotonergic abnormalities. in Proceedings of the National Academy of Sciences of the United States of America 2000 (PubMed)

  • Human BDNF ELISA Kit for Sandwich ELISA - ABIN365224 (2 Publications): Kim, Song, So, Lee, Song, Kim: Increase of circulating BDNF levels and its relation to improvement of physical fitness following 12 weeks of combined exercise in chronic patients with schizophrenia: a pilot study. in Psychiatry research 2015 (PubMed)

  • Human BDNF ELISA Kit for Sandwich ELISA - ABIN414355 (1 Publications): Li, Chen, Wang, Lü, Huan, Fang, Han, Ge, Chen: Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells. in Genetics and molecular research : GMR 2016 (PubMed)

Similar BDNF ELISA Kits

Application / Reactivity Human
ELISA 31 ELISA Kits

General

Antigen Brain-Derived Neurotrophic Factor (BDNF) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(34), (24), (22), (11), (8), (8), (7), (6), (6), (5), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 93.8-6000 pg/mL
Minimum Detection Limit 93.8 pg/mL
Application ELISA
Supplier Log in to see

Product Details BDNF ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of BDNF in human serum, plasma, and other biological fluids not being tested
Brand EasyTest™
Sample Type Serum, Plasma
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of human BDNF.
Sensitivity 1 pg/mL
Characteristics one wash
Components
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • human BDNF Mix-4x 9 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x8mL
  • Adhensive strip-2
  • Instruction manual-1.
Material not included 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Target details

Product Details BDNF ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name BDNF (BDNF ELISA Kit Abstract)
Background Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system.
Molecular Weight 14 kDa
Gene ID 627
NCBI Accession NP_001137277
UniProt P23560
Research Area Cardiovascular, Atherosclerosis, Alzheimer's Disease, Hormones
Pathways RTK Signaling

Application Details

Product Details BDNF ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 50 μL
Assay Time 1.5 h
Plate Strips (12 x 8)
Protocol This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for BDNF has been pre-coated onto a microplate. Standards, samples and human BDNF mix are pipetted into the wells and any BDNF present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BDNFbound in the initial step. The color development is stopped and the intensity of the color is measured.
Reagent Preparation 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 90 μL 1x Assay Diluent to prepare a 100 ng/mL standard. Gently vortex to mix.Take 30 μL BDNF standard into a tube, then add 470 μL 1x Assay Diluent to prepare a 6000 pg/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 150 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. human BDNF Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1490 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Sample Collection Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Sample Preparation Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:5.
Assay Procedure 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of BDNF Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 1 hour at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Calculation of Results 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Assay Precision intra CV<10%, inter CV <15%
Restrictions For Research Use only

Handling

Product Details BDNF ELISA Kit Target details Application Details Images back to top
Buffer 0.05 % Proclin 300
Preservative ProClin
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Storage 4 °C/-20 °C

Images

Product Details BDNF ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for BDNF ELISA Kit (Brain-Derived Neurotrophic Factor) (ABIN2506943) Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit